STRATEGIES OF EXTRACTING NUCLEIC ACIDS

Nucleic acids are bio-molecules which can be found in cells of all existing organisms, be it plants as well as animals. Nucleic acids plays a key role in the continuation of life in any existing organism. Nucleic acids contain DNA and RNA being a major components. DNA and RNA are essential components within the cells of the living plant structur that plays a major role in manufacture of necessary protein through a process of replication i actually. e. DNA replication.

Nucleic acids can be extracted via different methods, some of the procedures were demonstrated by the popular Swiss physician, Friedrick Miescher in 1869 when he ended up being determined to solve the problem of how does the basic theory of life works? Due to the fact nucleic acids are elements with unique characteristics, tends to make them to be able to be famous from other molecules hence capable of being extracted. Before these nucleic acids are being extracted, various other bio-molecules such as bacteriophages that i term as " impurities, " should be removed to help facilitate faster extraction of pure nucleic acids. DNA can be extracted using recombinant method. This method involves signing up for together two different types of DNA molecules from different varieties. This method ensures faster associated with specific DNA molecule within an organism since the two types associated with DNA materials are foreign to each other hence extraction of purified DNA molecule.

Several manual methods which includes centrifugal method can be used to ensure proper extraction of DNA as well as RNA bio-molecules, this method requires repeated centrifuge of protein materials such as blood along with other materials such as egg yolks, this is done in repeated occasions until the impurities in the healthy proteins materials are removed then the pure nucleic molecule is definitely remained with. However often purification of these proteins is essential in this method to ensure development of the best product.

Solution based method is a modern method which is popularly being adopted by several modern physician throughout the world, this method is a method that was developed by a series of famous medical professionals from older generation to date. this method involves removal of unwanted elements in the solution by make use of certain specific and more superior solutions. these solutions tend to be added to break the bond between and among the nucleic acid. This according to their unique method of bonding will result in the extraction of more filtered nucleic acids than the preceding methods as stated above.

Additionally an extraction kit comprising a bead that utilizes the principle of a magnet is employed to extract nucleic stomach acids from different types of the microorganisms this method is more efficient plus the cheapest method of all the other techniques. The magnetic rod solely attract to certain specific DNA and RNA bio-molecules hence production of a pure nucleic molecules.

Other procedures such as anion exchange supplies, Ion exchange chromatography and also affinity are used to extract nucleic acids, these methods are mostly laboratory methods which works by using certain principles and a likewise follow strict procedure. there is an automated method of extraction which will utilizes a very expensive tool that can easily extract nucleic acids.

automated nucleic acid extraction
automated nucleic acid extraction
automated nucleic acid extraction

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